@article{MiguelMourato2014,
	author={Miguel Mourato and Luisa Louro Martins and Sergio Baptista and Rafaela Reis and Florbela de Oliveira Carvalheiro Esteves Amaro and Andre M. Almeida and Manuel Pedro Salema Fevereiro and Ann Cuypers},
	journal={Acta Physiologiae Plantarum},
	month={march},
	note={The response of tobacco plants genetically engineered with the AtTPS1
   gene to stress induced by excess Cu and Cd was evaluated in hydroponic
   solution (100 and 400 mu M Cu and 50 and 200 mu M Cd) after a 48 h
   exposure. Two transgenic lines, transformed with the AtTPS1
   (trehalose-6-phosphate synthase) gene from Arabidopsis, with different
   levels of trehalose-6-phosphate synthase expression (B5H, higher and
   B1F, lower), and a wild type (WT) were investigated. Protein content,
   antioxidative enzymes (CAT, POD, SOD, and APX), glucose, fructose, lipid
   peroxidation, hydrogen peroxide and Cd and Cu contents were determined
   in leaves. The two transgenic lines were differently influenced by Cd
   and Cu exposure as they induced a different antioxidant enzymatic
   defense response. B1F and B5H plants showed a better acclimation to Cd
   and excess Cu compared to WT. Furthermore B1F was more tolerant than B5H
   to Cd and excess Cu. B1F accumulated less Cd and Cu in leaves, probably
   due to a more efficient exclusion mechanism. Catalase was shown to be
   the most important enzyme in the antioxidative system of these plants.},
	pages={755-765},
	series=3,
	title={Response to oxidative stress induced by cadmium and copper in tobacco plants (Nicotiana tabacum) engineered with the trehalose-6-phosphate   synthase gene (AtTPS1)},
	volume=36,
	year=2014,
}