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Publicação
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Título:
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Response to oxidative stress induced by cadmium and copper in tobacco plants (Nicotiana tabacum) engineered with the trehalose-6-phosphate synthase gene (AtTPS1)
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Tipo:
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Artigo de Revista
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Autor(es):
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Miguel Mourato
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Luisa Louro Martins
,
Sergio Baptista
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Rafaela Reis
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Florbela de Oliveira Carvalheiro Esteves Amaro
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Andre M. Almeida
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Manuel Pedro Salema Fevereiro
,
Ann Cuypers
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Resumo:
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The response of tobacco plants genetically engineered with the AtTPS1
gene to stress induced by excess Cu and Cd was evaluated in hydroponic
solution (100 and 400 mu M Cu and 50 and 200 mu M Cd) after a 48 h
exposure. Two transgenic lines, transformed with the AtTPS1
(trehalose-6-phosphate synthase) gene from Arabidopsis, with different
levels of trehalose-6-phosphate synthase expression (B5H, higher and
B1F, lower), and a wild type (WT) were investigated. Protein content,
antioxidative enzymes (CAT, POD, SOD, and APX), glucose, fructose, lipid
peroxidation, hydrogen peroxide and Cd and Cu contents were determined
in leaves. The two transgenic lines were differently influenced by Cd
and Cu exposure as they induced a different antioxidant enzymatic
defense response. B1F and B5H plants showed a better acclimation to Cd
and excess Cu compared to WT. Furthermore B1F was more tolerant than B5H
to Cd and excess Cu. B1F accumulated less Cd and Cu in leaves, probably
due to a more efficient exclusion mechanism. Catalase was shown to be
the most important enzyme in the antioxidative system of these plants.
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Palavras-chave:
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Antioxidative enzymes; Cadmium; Copper; Oxidative stress; Tobacco
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Página inicial:
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755
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Página final:
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765
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Revista:
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Acta Physiologiae Plantarum - 36 - 3 [2014]
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Editora:
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Springer
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URL:
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http://link.springer.com/article/10.1007%2Fs11738-013-1453-0
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Documentos
Não existem documentos.
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