Floral development and determination of a gene function.

4 Novembro 2020, 14:00 • Margarida Maria Pedro Rocheta

Functional Genomics: Forward and reverse genetics- advantages and limitations. Interference RNA (RNAi). Mechanisms used by RNAi and miRNA.

Techniques for RNA analysis, with particular detail for the in situ hybridization protocol. Technique of preparing vegetable tissues for microtome cutting. Aspects of the preparation of RNA probes; various types of controls to be used. Type of final signal and its interpretation. Demonstrative video. Practical example.